Racing Pigeon DNA Test for Flight Ability

Racing Pigeon DNA Test for Flight Ability Instructions

Racing Pigeon DNA Test for Flight Ability uses whole genome and RNA sequencing data to test racing pigeons for superior flying and orientation abilities.

【Racing Pigeon DNA Test for Flight Ability Product Name】

General name: Racing Pigeon DNA Test for Flight Ability (PCR-fluorescence probe method)

【Racing Pigeon DNA Test for Flight Ability Expected Use】

This kit is used for qualitative genetic testing of pigeon flight capabilities and can predict pigeon flight capabilities.

【Racing Pigeon DNA Test for Flight Ability detection principle】

Design specific primers and probes based on the pigeon's flight ability genes, and use fluorescence quantitative PCR technology to detect the flight ability genes by detecting the release and intensity of fluorescence.

【Composition of Racing Pigeon DNA Test for Flight Ability Kit】

This product consists of specific primer probes, Taq enzymes and reaction buffer.

【Racing Pigeon DNA Test for Flight Ability Main components】

【Racing Pigeon DNA Test for Flight Ability Storage Conditions and Validity Period】

-20℃ is safe from light and is 12 months; avoid repeated freeze-thawing.

【Racing Pigeon DNA Test for Flight Ability Sample Requirements】

1.Feathers: Collect 2 feathers on the chest, abdomen or legs, and avoid using naturally falling feathers.

2.Whole blood: Use EDTA as an anticoagulant, and avoid using heparin to anticoagulant.It is required to use fresh whole blood, or store it at 2 to 8℃ for no more than 7 days, or store it below -20℃ for no more than one month.Try to avoid repeated freeze-thawing.

【Racing Pigeon DNA Test for Flight Ability Test Method】

1.Sample processing and nucleic acid extraction, refer to the relevant operating instructions for specific operations.

(1) Add 2-4 feathers to the 0.2ml PCR tube, add Reagent A 20ul (note the flooding sample), and heat at 99°C for 20 minutes;

(2) Add Reagent B 100ul to the Pcr tube in the previous step, centrifuge at 5000rpm for 30 seconds-1 minute, and set aside;

2.The instrument is ready to turn on each instrument and check whether it is operating normally.

3.Item preparation: centrifuge, ice box, pipette, suction head; reagent preparation (used without compartmentation specification): Thaw and mix the PCR reaction solution, and then centrifuge instantly and aliquot 14 µL/tube.

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4.PCR amplification conditions (reaction system is 20μL)

5.Quantitative fluorescence detection

Result judgment

【Limitations of Racing Pigeon DNA Test for Flight Ability Test Method】

This method is only applicable to genetic testing of pigeon flight ability.

【Racing Pigeon DNA Test for Flight Ability Product Performance Indicators】

1.Specificity: Test specific reference products, and all reference products have negative test results.

2.Accuracy: Detection of reference products of different genotypes within the detection range can detect the corresponding genotype.

3.Minimum detection limit: should not be higher than 5ng/µL.

4.Repeatability: Repeatable reference products of different genotypes within the detection range.Each reference product is tested 10 times, and the results can be accurately classified, and the coefficient of variation (CV) of the corresponding detection result Ct value should be ≤5.0%.

5.Validity period: The kit is stored in -20 away from light, and the validity period is 12 months.

【Precautions for Racing Pigeon DNA Test for Flight Ability】Please read the instruction manual carefully before the experiment

1.This kit only detects the in vitro gene of pigeon flight ability.The relevant laboratory management standards shall be strictly implemented in accordance with the management regulations of the relevant gene amplification detection laboratories issued by the administrative department.

2.The kit should be transported and stored at low temperature.Please centrifuge temporarily before using the reagents in the kit.

3.During testing, the sample extraction must be carried out in the biosafety cabinet, and attention should be paid to lateral contamination when used.

4.The experiment should be strictly divided into sections: Zone 1: Reagent preparation area - prepare the reagents required for amplification;

Zone 2: Sample treatment area—treatment of samples to be tested and reference samples;

The third area: PCR amplification zone—PCR amplification detection.

This product is a disposable product.Wastes such as kits and samples after use should be treated as sources of infection in accordance with relevant national laws and regulations.